本研究旨在探討利用結合溶膠凝膠法與水熱法，合成不同構型之二氧化鈰/二氧化鈦(CeO2/TiO2)奈米材料，並探究其可否做為選擇性富集磷酸化胜肽分子之奈米探針。當分析所合成的四種不同構型，包含立方體(cube)、棒狀(rod)、球形(sphere)與八面體(octahedral)的CeO2/TiO2)奈米材料時，實驗結果顯示立方體構型之CeO2/TiO2)奈米材料對於磷酸化胜肽的富集有著較為良好的靈敏度與專一性。除了最低可偵測到2 femtomole之低豐度β-酪蛋白(β-casein)之磷酸化胜肽外，亦可於牛血清蛋白(BSA)和β-casein以莫爾數比1000:1的比例混合下，有效偵測屬於-casein的磷酸化胜肽片段，並且使用標準磷酸化胜肽進行回收率測試與最大磷酸化胜肽吸附容量實驗時，分別可以達到88.9%的回收率與100 mg/g的最大吸附容量。此外，當進行真實樣品如血清與奶粉的磷酸化胜肽分析時，此奈米探針亦能選擇性的分離出7個與4個之磷酸化胜肽片段，代表此奈米材料可對於複雜生物樣品中磷酸化胜肽偵測具有良好的潛力。

In this study, CeO2/TiO2 nanomaterial with different shape have been prepared through the sol–gel method under hydrothermal treatment, to be used as an affinity probe for the enrichment of phosphopeptides. Among the four shape-controlled nanostructures (i.e. cube, nanorod, sphere, and octahedral), the mass spectrometric results reveal that CeO2/TiO2 cube nanocrystals provide the superior performance on capturing phosphopeptides from tryptic digests of β-casein. Under the optimized condition for phoshpopeptides enrichment, the as-prepared nanomaterial possess high detection sensitivity (2 fmol) for β-casein tryptic phosphopeptides, high selectivity (a molar ratio of bovine serum albumin digest and β-casein digest of 1000:1), reasonable capture recovery (88.9%), high capture capacity (100 mg/g) and good repeatability. This technique demonstrates its potential by effectively enriching phosphopeptides in the complex biological specimens. Seven and four phosphopeptides were selectively enriched from the milk powder digest and human serum digest, respectively.

第一章 緒論
第二章 研究目標
第三章 研究內容
第四章 實驗結果
第五章 結論
第六章 參考文獻

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